Supplementary Information for

Dokshin GA, Davis GM, Sawle AD, Eldridge MD, Nicholls PK, Gourley TE, Romer KA, Molesworth LW, Tatnell HR, Ozturk AR, de Rooij DG, Hannon GJ, Page DC, Mello CC, Carmell MA GCNA interacts with spartan and topoisomerase II to regulate genome stability Dev Cell epub Dec 4 (2019)

Figures

  • Supplementary Figure 1   Gcna-1 mutant germ cells exhibit normal proliferation, RNA granule morphology, and slightly elevated apoptosis relative to wildtype
  • Supplementary Figure 2   No drug genotoxic controls and chromatin bridging
  • Supplementary Figure 3   Integrative Genomics Viewer (IGV) visualization of alignments of paired-end Illumina reads to the reference genome sequence at the C. elegans unc-58 locus
  • Supplementary Figure 4   GCNA expression and wild-type and Gcna- mutant responses to UV irradiation in mouse ES cells
  • Supplementary Figure 5   Wild-type GCNA expression and Gcna- mutant chromosome condensation, synapsis, and DNA damage defects
  • Supplementary Figure 6   Cumulative distribution curves of the distance between two MLH1 foci in wild-type and mutant bivalents (Chromosomes 11-13)

    Tables

  • Supplementary Table 1   Alignment Metrics for C. elegans Genome Sequencing, Related to STAR Methods
  • Supplementary Table 2   Homozygous Deletions in gcna-1 and dvc-1 Mutant Backgrounds from C. elegans Genome Sequencing Called by VarScan
  • Supplementary Table 3   Structural Variant Calls in gcna-1 and dvc-1 Mutant Backgrounds from C. elegans Genome Sequencing
  • Supplementary Table 4   Discordant Uniquely Mapped Read Pairs in gcna-1 and dvc-1 Mutant Backgrounds from C. elegans Genome Sequencing
  • Supplementary Table 5   Proteins Identified by Mass Spectrometry Following GCNA Immunoprecipitation from Mouse ESCs
  • Supplementary Table 6   RNA Sequencing Data from Wild-Type and Gcna-Mutant Juvenile Mouse Testes