Supplementary Information

Supplementary Information for

Pfaltzgraff NG, Liu B, de Rooij DG, Page DC, Mikedis MM

Destabilization of mRNAs enhances competence to initiate meiosis in mouse spermatogenic cells

Supplementary File: Supplementary Materials and Methods; Supplementary Figures S1-S14
Table S1: Supplementary table and stats by figure panel
Table S2: scRNA-seq analysis of WT germ cell clusters from P15 testis
Table S3: scRNA-seq differential expression analysis (DEA)
Table S4: scRNA-seq DEA WT v. KO Gene Ontology analysis
Table S5: Associated cell cycle phase of MEIOC-downregulated genes
Table S6: MEIOC, YTHDC2, and RBM46 target analysis
Table S7: mRNA stability and transcriptional rate from bulk RNA-seq data
Table S8: Number of cells per scRNA-seq cluster in P15 testes
Table S9: bulk RNA-seq differential expression analysis, WT vs. Meioc KO
Table S10: Cell cycle analysis of bulk RNA-seq Meioc and Stra8 datasets
Table S11: bulk RNA-seq Gene Ontology analysis
Table S12: Reanalysis of E2f6 and Mga data from embryonic stem cells
Table S13: MEIOSIN quantification in WT v Meioc KO