# Supplemental File S2 - primer and nucleotide sequences used # in miRNA transfection experiments # ----------------------------------------------------------- # EIF1AX_3utr... : primers for cloning EIF1AX 3' UTR # EIF1AY_3utr... : primers for cloning EIF1AY 3' UTR # EIF1AX_mut... : primers for shuffling miR-1 target site in EIF1AX # EIF1AY_add... : primers for repairing miR-1 target site in EIF1AY # mir-[1|124]_duplex : oligonucleotide sequences for constructing # miR-1 and miR-124 duplexes >EIF1AX_3utr_start_fwd (5‰Ûª Pme1 site) (for cloning) GTTTAAACGGCCCCATTGAACTCAACATTTTACATTCC >EIF1AX_3utr_1015bp_rev (3‰Ûª Not1 site) (for cloning) ATCGAGCGGCCGCTCAGAGACCATCTTTTCACTGAGTTGT >EIF1AY_3utr_start_fwd (5‰Ûª Pme1 site) (for cloning) GTTTAAACCCGGTCATTGAACCAAGTGTTTTTACATGAC >EIF1AY_3utr_rev (3‰Ûª Not1 site) (for cloning) ATCGAGCGGCCGCTCATATAGAATTTATTATGAAATTGCTTTTCATT >EIF1AX_mut_miR1site_fwd (for mutagenesis) TTGAACTCAACATTTTTGTTTCCATCTTTTCTGAAGATTGTCCT >EIF1AX_mut_miR1site_rev (for mutagenesis) AGGACAATCTTCAGAAAAGATGGAAACAAAAATGTTGAGTTCAA >EIF1AY_add_miR1site_fwd (for mutagenesis) ACCAAGTGTTTTTACATTCCAAGTTCTCTGAGGAT >EIF1AY_add_miR1site_rev (for mutagenesis) ATCCTCAGAGAACTTGGAATGTAAAAACACTTGGT >miR-1_duplex_fwd (for transfection) UGGAAUGUAAAGAAGUAUGUA >miR-1_duplex_rev (for transfection) CAUACUUCUUUACAUUCAAUA >miR-124_duplex_fwd (for transfection) UAAGGCACGCGGUGAAUGCCA >miR-124_duplex_rev (for transfection) GCAUUCACCGCGUGCCUUAAU SupportCancel