Supplementary Information for
Dawlaty MM, Ganz K, Powell BE, Hu YC, Markoulaki S, Cheng AW, Gao Q, Kim J, Choi SW, Page DC, Jaenisch R.
Tet1 is dispensable for maintaining pluripotency and its loss is compatible with embryonic and postnatal development.
Cell Stem Cell 9(2), 166 (2011)
- Figure S1 sion of Tet2 and Tet3 in Tet1 knockout ES cells along with shRNA-mediated depletion of Tet2 in Tet1 Knockout ES cells and its effects on 5hmC and expression of pluripotency markers. (Related to quantification of 5hmC in Figure 2).
- Figure S2 List of up or down regulated genes in Tet1 knockout ES cells. (Related to gene expression profile and GO analysis in Figure 2).
- Figure S3 Characterization of Tet1 knockout ES cells when cultured on gelatin along with in vitrodifferentiation of Tet1 knockout ES cells to EBs and NPs. (Related to pluripotency marker expression and in vitrodifferentiation defects in Tet1 knockout ES cells explained in Figure 3).
- Figure S4 Analysis of blood from Tet1 knockout mice for CBC, differentials and liver function. (Related to generation and characterization of Tet1 knockout mice in Figure 4).